IMR Press / FBE / Volume 11 / Issue 1 / DOI: 10.2741/E843

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Fisetin protects against rotenone-induced neurotoxicity through signaling pathway
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1 Department of Biochemistry, Research and Development Centre, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India
2 Department of Biochemistry, Indian Academy Degree College, Autonomous, Bengaluru- 560043, Karnataka, India
3 Department of Biochemistry, M.G.R. College, Hosur-635130, Tamil Nadu, India
Send correspondence to: Rajamurugan Ramachandran, Department of Biochemistry, Research and Development Centre, Bharathiar University, Coimbatore- 641046, Tamil Nadu, India, Tel: 914344-261004, 261018, Fax: 914344-261570, E-mail: malathianand26@gmail.com
Front. Biosci. (Elite Ed) 2019, 11(1), 20–28; https://doi.org/10.2741/E843
Published: 1 January 2019
Abstract
ABSTRACT

The present study was designed to evaluate the protective effect of fisetin against rotenone induced toxicity in SH-SY5Y neuroblastoma cellular modelof Parkinson's disease (PD). SH-SY5Y neuroblastoma cells were treated with fisetin (5µM) 2 hr prior to being treated with rotenone (100 nM). Following the exposure ofSH-SY5Ycells torotenone, there was marked decreased cell viability, increased oxidative stress, activation of caspase-3 and apoptosis (dual staining, expressions of pro-apoptotic and anti-apoptotic indices). However pretreatment with fisetin significantly and dose-dependently alleviated rotenone induced cytotoxicity and oxidative stress inSH-SY5Ycells. Moreover, fisetin attenuatedrotenoneinduced toxicity by down-regulatingBax, caspases-3 protein expression and up-regulating protein expression of Bcl-2, p38/JNK-MAPK and PI3K, Akt, GSK-3β pathways. Collectively, these results suggest that fisetin could prevent therotenone-induced neurotoxicity via various signaling pathways.

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