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- Academic Editor
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†These authors contributed equally.
This is an open access article under the CC BY 4.0 license.
Background: Pyroptosis plays a crucial role in
anti-tumor immunity and in formation of the immune microenvironment. However,
whether pyroptosis is involved in the progression of
clear cell renal cell carcinoma (ccRCC) is still unclear. Personalized treatment
of ccRCC requires detailed molecular classification to inform a specific therapy.
Methods: Molecular subtyping of ccRCC was performed based on consensus
clustering of pyroptosis-related genes. The characteristics of these molecular
subtypes were explored at the genome, transcriptome and protein levels.
Single-cell RNA sequencing and CIBERSORT analysis were used to analyse the immune
microenvironment of ccRCC, while Lasso regression was used to develop a
prediction model based on hub genes. Expression of the pyroptosis-related gene
GSDMB was also investigated at the tissue and cellular levels.
Results: Two molecular subtypes were identified based on the clustering
of pyroptosis-related genes. Cluster 1 was associated with activation of
classical oncogenic pathways, especially the angiogenesis pathway. Cluster 2 was
associated with activation of immune-related pathways and high levels of
immunosuppressive cells, exhausted CD8