Background: Exposure to low dose rate (LDR) radiation may accelerate
aging processes. Previously, we identified numerous LDR-induced pathways involved
in oxidative stress (OS) and antioxidant systems, suggesting that these pathways
protect against premature senescence (PS). This study aimed to investigate if
there are differences between young replicative senescent (RS) and PS cells
considering DNA repair kinetics, OS, and DNA damage localized in the telomeres.
Methods: We established PS cells by culturing and passaging young
primary fibroblasts exposed to LDR. Then, RS cells were established by culturing
and passaging young fibroblasts until they stopped proliferating. Senescence was
characterized by analyzing telomere length and senescence-associated
Announcements
Open Access
Original Research
Oxidative Stress Levels and DNA Repair Kinetics in Senescent Primary Human Fibroblasts Exposed to Chronic Low Dose Rate of Ionizing Radiation
Traimate Sangsuwan1, Ali Pour Khavari1, Evelina Blomberg1, Tajanena Romell1, Paulo Roberto D'auria Vieira De Godoy1, Mats Harms-Ringdahl1, Siamak Haghdoost1,2,3,*
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1
Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden
2
CIMAP/ARIA team, University of Caen Normandy, 14000 Caen, France
3
Advanced Resource Center for HADrontherapy in Europe (ARCHADE), 14000 Caen, France
*Correspondence: siamak.haghdoost@su.se (Siamak Haghdoost)
Front. Biosci. (Landmark Ed) 2023, 28(11), 296;
https://doi.org/10.31083/j.fbl2811296
Submitted: 15 June 2023 | Revised: 9 September 2023 | Accepted: 13 September 2023 | Published: 24 November 2023
(This article belongs to the Special Issue Influence of Radiation Quality on Cellular, Molecular and Inflammatory Responses in Normal and Malignant Human Cells)
Copyright: © 2023 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract
Keywords
radiation
chronic radiation
low dose rate
premature senescence
replicative senescence
DNA repair
radiotherapy
oxidative stress
hMTH1
telomere length
extracellular 8-oxo-dG
Funding
The Swedish Radiation Safety Authority, SSM
Figures
Fig. 1.