IMR Press / FBL / Volume 27 / Issue 10 / DOI: 10.31083/j.fbl2710279
Open Access Original Research
Expression and Predictive Functional Profiles of MicroRNAs Data in Vascularized Composite Allotransplantation Acute Rejection
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1 Department of Plastic Surgery, The Third Xiangya Hospital, Central South University, 410083 Changsha, Hunan, China
2 Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 200030 Shanghai, China
3 Department of Orthopedics, The Affiliated Huai’an Hospital of Xuzhou Medical University and The Second People’s Hospital of Huai’an, 223022 Huai’an, Jiangsu, China
4 College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 201620 Shanghai, China
5 Department of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, 200127 Shanghai, China
*Correspondence: (Lincai Ye); (Jianda Zhou); (Shoubao Wang)
These authors contributed equally.
Academic Editor: Rivka Ofir
Front. Biosci. (Landmark Ed) 2022, 27(10), 279;
Submitted: 24 May 2022 | Revised: 11 August 2022 | Accepted: 24 August 2022 | Published: 30 September 2022
(This article belongs to the Special Issue Signal Transduction and Gene Expression for Modeling Human Diseases)
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: This study aimed to investigate the mechanisms of acute rejection for vascularized composite allotransplantation (VCA) using microRNAs (miRNAs) differential expression in a VCA animal model. Methods: Brown Norway rats were used as transplant donors and Lewis rats as VCA receptors. The changes were divided into different stages before and after transplantation in Lewis rats, and all appearance changes were recorded. Also, histological evaluations were performed on all recipients, and the expression of microRNAs was analyzed when acute immune rejection occurred. Then, we used GO and KEGG Pathway enrichment analyses to predict miRNA targets. Finally, differentially expressed miRNAs were detected by RT-qPCR. Results: Compared to pre-operation, 22 miRNAs were differentially expressed after operations. Among them, nine were upregulated, and 13 were downregulated in skin tissues. The RT-qPCR results revealed that rno-miR-340-5p and rno-miR-21-5p were significantly upregulated and enriched in the PI3K-Akt signaling pathway. Moreover, rno-miR-145-5p and rno-miR-195-5p were significantly downregulated, and most of their target genes were enriched in the Hippo signaling pathway. The histological evaluations showed that, after VCA, the skin tissue presented severe acute rejection. Conclusions: The miRNAs rno-miR-340-5p, rno-miR-21-5p, rno-miR-145-5p, and rno-miR-195-5p were significantly regulated during VCA acute rejection, when the four miRNAs analyses were done on skin biopsies. These miRNAs might be potential biomarkers for objective, early, and minimally invasive rejection diagnosis.

vascularized composite tissue allograft 1
miRNAs 3
acute rejection 4
2018ZX10303502/National Science and Technology Major Projects of China
2019T120347/Postdoctoral Science Foundation
82002065/Natural Science Foundation of China
Fig. 1.
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