IMR Press / FBE / Volume 14 / Issue 1 / DOI: 10.31083/j.fbe1401007
Open Access Original Research
Polyclonal antibodies against potato spindle tuber viroid RNA
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1 Belozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russian Federation
2 Federal Research Centre “Fundamentals of Biotechnology", Russian Academy of Sciences, 119071 Moscow, Russian Federation
3 Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russian Federation
*Correspondence: (Yuri Drygin)
Academic Editor: Baohong Zhang
Front. Biosci. (Elite Ed) 2022, 14(1), 7;
Submitted: 19 September 2021 | Revised: 10 January 2022 | Accepted: 13 January 2022 | Published: 4 March 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Ribonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with synthesized conjugate viroid RNA-lysozyme. Analyses of polyclonal mouse and rabbit antisera as well as estimates of antibody specificity were performed by dot-Enzyme Linked Immunosorbent Assay (ELISA), sandwich ELISA, and northern immunoblotting. Antiserum obtained showed strong cross-reactions with cellular RNA. The viroid polyclonal antibody cross-reactions with cellular RNAs were depleted via titration antibodies by the plant cellular or commercial yeast RNA. We successfully used antibodies against the viroid RNA-lysozyme antigen to detect the wild-type potato viroid and diagnose potato viroid infection. We presume that intrinsic cross-reactions of RNA antibodies are potentially dangerous after nucleic acid vaccination. Research into the specificity of antibodies against viral RNAs is underway.

potato spindle tuber viroid
immune northern blotting
nucleic acid vaccine
Fig. 1.
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