Background: The African swine fever (ASF) virus (ASFV) and ASF-like
viral sequences were identified in human samples and sewage as well as in
different water environments. Pigs regularly experience infections by the ASFV.
The considerable stability of the virus in the environment suggests that there is
ongoing and long-term contact between humans and the ASFV. However, humans
exhibit resistance to the ASFV, and the decisive factor in developing infection
in the body is most likely the reaction of target macrophages to the virus.
Therefore, this study aimed to characterize the responses of human macrophages to
the virus and explore the distinct features of the viral replication cycle within
human macrophages. Methods: The ASFV Armenia/07 strain was used in all
experiments. In this study, quantitative real-time polymerase chain reaction
(qRT-PCR) was used to determine the ASFV gene expression; flow cytometry analysis
was performed to evaluate the effects of the inactive and active ASFV (inASFV and
aASFV) treatments on the phenotype of THP-1-derived macrophages (M
Announcements
Open Access
Original Research
Infection of Human Macrophage-Like Cells by African Swine Fever Virus
Zaven A. Karalyan1,*, Susanna A. Ghonyan2, Davit A. Poghosyan2, Lina H. Hakobyan1, Hranush R. Avagyan1,3, Aida S. Avetisyan1,3, Liana O. Abroyan1, Arpine A. Poghosyan1, Sona A. Hakobyan1, Gayane P. Manukyan2
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1
Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, 0014 Yerevan, Armenia
2
Laboratory of Molecular and Cellular Immunology, Institute of Molecular Biology NAS RA, 0014 Yerevan, Armenia
3
Experimental Laboratory, Yerevan State Medical University after M. Heratsi, 0025 Yerevan, Armenia
*Correspondence: zkaralyan@yahoo.com (Zaven A. Karalyan)
Front. Biosci. (Landmark Ed) 2024, 29(4), 164;
https://doi.org/10.31083/j.fbl2904164
Submitted: 21 September 2023 | Revised: 15 January 2024 | Accepted: 18 February 2024 | Published: 23 April 2024
Copyright: © 2024 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract
Keywords
African swine fever virus
THP-1-derived human macrophages
gene transcription
cytometry
TLRs
LAMP-1
Figures
Fig. 1.