IMR Press / FBL / Volume 28 / Issue 9 / DOI: 10.31083/j.fbl2809202
Open Access Original Research
Transcriptome Analysis Reveals Long Non-Coding RNAs Involved in Shade-Induced Growth Promotion in Pinellia ternata
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1 Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, 235000 Huaibei, Anhui, China
*Correspondence:; (Jianping Xue)
Front. Biosci. (Landmark Ed) 2023, 28(9), 202;
Submitted: 29 November 2022 | Revised: 9 February 2023 | Accepted: 13 February 2023 | Published: 14 September 2023
Copyright: © 2023 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: High temperature and drought environments are important limiting factors for Pinellia ternata growth, whereas shading can promote growth by relieving these stresses. However, the mechanism of growth promotion by shading in P. ternata is unknown. Long non-coding RNAs (lncRNAs) play important roles in the plant’s growth and environmental response, but few analyses of lncRNAs in P. ternata have been reported. Methods: We performed lncRNAs analysis of P. ternata in response to shading using RNA-seq data from our previous studies. A total of 13,927 lncRNAs were identified, and 145 differentially expressed lncRNAs (DELs) were obtained from the comparisons of 5 days shade (D5S) vs. 5 days of natural light (D5CK), 20 days of shade (D20S) vs. 20 days of natural light (D20CK), D20S vs. D5S, and D20CK vs. D5CK. Of these, 119 DELs (82.07%) were generated from the D20S vs. D20CK comparison. Results: Gene ontology (GO) analysis indicated that the reactive oxygen (ROS) metabolism and programmed cell death (PCD) processes might regulate shade-induced growth promotion. The “signal transduction” and “environmental adaptation” in the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used for lncRNA-mRNA regulatory network construction and showed that the lncRNAs might mediate P. ternata growth by regulating ROS accumulation and light signals. Conclusions: This study explores lncRNAs’ functions and regulatory mechanisms related to P. ternata growth and lays a foundation for further research on P. ternata.

Pinellia ternata
long noncoding RNAs
growth promotion
81803665/National Natural Science Foundation of China
81573518/National Natural Science Foundation of China
KJ2021A0532/Project of Natural Science Research of Universities in Anhui Province
KJ2021A0533/Project of Natural Science Research of Universities in Anhui Province
2022AH010029/Excellent Scientific Research and Innovation Team of University in Anhui Province
NELCOF20210102/Open project of National and local Joint Engineering Laboratory of Anti-stress Breeding and Disaster Reduction
Fig. 1.
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