CAR-T cell structures. First, second, third, fourth and fifth generation of CARs. ScFv, single chain variable fragment; VH-variable heavy chain; VL, variable light chain, hinge, transmembrane, CD28, 4-1BB or other costimulatory domains, CD3 zeta activation domain. The first generation of CAR included one CD3 zeta activation domain; the second generation of CAR included one costimulatory domain and one CD3 zeta activation domain. The third generation of CAR includes two costimulatory domains (for example, CD28 and 4-1BB) and one activation CD3 zeta domain. The fourth generation of CAR included second generation of CAR with secreted cytokine or chemokine protein. The fifth generation of CAR included additional expression of receptor domain for example IL-2 receptor chain beta stimulating STAT3/STAT5 signaling.