IMR Press / FBE / Volume 4 / Issue 6 / DOI: 10.2741/e522

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

TGF-beta 1/Smad3 expression and its effects on carotid intimal hyperplasia

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1 Department of Geriatrics, Shanghai Ninth People’s Hospital Affiliated Shanghai Jiao Tong University School of Medicine, China

Academic Editor: Xinyu Wang

Front. Biosci. (Elite Ed) 2012, 4(6), 2022–2028; https://doi.org/10.2741/e522
Published: 1 January 2012
Abstract

We investigated expression of TGF-beta 1/Smad3 after balloon injury in rat carotid arteries as well as the effects of blocking the TGF-beta 1/smad3 signaling pathway on carotid intimal hyperplasia. Rats were randomly divided into control group (C group) and injury group (S group). The latter were randomly divided into intervention group (antisense Smad3 adenovirus vector transfection after balloon injury) and blank control group (blank adenovirus vector transfection after balloon injury). Expression of Smad3 mRNA 1 d, 3 d, 1 week, 2 weeks and 1 month after injury and intima/media thickness ratios 1 d, 2 weeks and 3 months after injury in the intervention group were significantly lower than those in the injury group. Ki67 expression in the intervention group was inhibited as shown in immunohistochemistry studies. These results demonstrated that antisense Smad3 adenovirus vector transfection can block TGF-beta 1/Smad3 signal transduction and thus inhibit intimal hyperplasia.

Keywords
Balloon injury
Intimal hyperplasia
TGF-beta 1/Stand3 signaling pathway
Antisense Smad3
Adenovirus
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