ADAR1 promotes HBV replication through its deaminase domain

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Article

Lin Yuan^1,2, Qianying Jia³, Shenyong Yang¹, Nur Fazleen Binti Idris¹, Yimin Li¹, Yang Wang¹, Ya Zhao², Jieli Hu⁴, Yuan Hu⁴, Ailong Huang⁴, Zeng Tu^1,4,*

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¹ Department of Microbiology, College of Basic Medical Sciences/Molecular Medicine and Tumor Research Center, Chongqing Medical University, Chongqing 400016, China

² Department of Clinical Laboratory, The First Hospital of Xi'an, Xi'an 710002, China

³ Department of Infectious Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

⁴ Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China

Send correspondence to: Ailong Huang or Zeng TU, Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, NO.1 YiXueYuan Road, Yuzhong District, Chongqing, 400016, P.R. China, Tel: 862368486780, Fax: 862368486780, E-mail: tuzeng@cqmu.edu.cn

Front. Biosci. (Landmark Ed) 2020, 25(4), 710–721; https://doi.org/10.2741/4830

Published: 1 January 2020

(This article belongs to the Special Issue Leader sequences of coronavirus are altered during infection)

Abstract

Adenosine deaminase acting on RNA1 (ADAR1) is an RNA editing enzyme that modulates the replication of several viruses. Here, we provide evidence showing that ADAR1 stimulates hepatitis B virus (HBV) DNA replication in hepatocellular carcinoma cell lines that are transiently or stably-transfected with HBV. We show that overexpression of ADAR1 promotes the replication of all four HBV genotypes (A, B, C, and D). Furthermore, analysis by mutagenesis shows that RNA editing region, and to a lesser content, RNA binding region of ADAR is responsible for the promotion of replication. Together, these data show that ADAR1 stimulates HBV replication.

Keywords

Adenosine Deaminase

RNA1

ADAR1

Hepatitis B virus

RNA Editing

Replication

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