IMR Press / JIN / Volume 22 / Issue 1 / DOI: 10.31083/j.jin2201021
Open Access Original Research
iTRAQ and PRM-Based Comparative Proteomic Profiling of the Hippocampus in Rat Models of Epilepsy
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1 Department of Forensic Medicine, Chongqing Medical University, 400010 Chongqing, China
2 Faculty of Medical Technology, Chongqing Medical and Pharmaceutical College, 401331 Chongqing, China
3 Chongqing Engineering Research Center of Pharmaceutical Sciences, 401331 Chongqing, China
*Correspondence: 102870@cqmu.edu.cn (Minzhu Zhao); 100390@cqmu.edu.cn (Jianbo Li)
These authors contributed equally.
Academic Editor: Kuei-Sen Hsu
J. Integr. Neurosci. 2023, 22(1), 21; https://doi.org/10.31083/j.jin2201021
Submitted: 24 May 2022 | Revised: 17 July 2022 | Accepted: 18 July 2022 | Published: 16 January 2023
(This article belongs to the Special Issue Neuroscience and Epilepsy: History and Potential Lessons)
Copyright: © 2023 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Background: Epilepsy is a disease caused by paroxysmal abnormal supersynchronous electrical activity of brain neurons, and it is also one of the most common illnesses in neurology. Among the causes, hippocampal sclerosis may be one of the main causes of temporal lobe epilepsy. However, the pathogenesis of hippocampal sclerosis in epilepsy remains unclear. Methods: We established an epilepsy model by intraperitoneal injection of pentetrazol (PTZ) into Sprague-Dawley rats, and applied isobaric tags for relative and absolute quantitation (iTRAQ) technology to identify differentially expressed proteins (DEPs) in the hippocampus. We quantified a total of 3782 proteins. DEPs were defined as proteins with a fold change >1.2 (or <0.83) and a Q value (p-adjusted) <0.05. Results: Comparing the epilepsy group and the control group, we identified 170 DEPs, comprising 109 upregulated and 61 downregulated proteins. According to bioinformatics analysis, the DEPs were primarily involved in long-term potentiation, the calcium signalling pathway, aldosterone synthesis and secretion, carbon metabolism, and dopaminergic synapses. Four of these proteins were validated using parallel reaction monitoring (PRM), including Glud1, Atp1a2, Prkcg and Arpc3. Conclusions: Our research results may provide further insight into the molecular pathology of hippocampal injury in epilepsy.

Keywords
epilepsy
hippocampus
proteomic
iTRAQ
PRM
Funding
cstc2019jcyj-msxmX0455/Basic and Frontier Technological Research Program of Chongqing
KJQN201902804/Scientific and Technological Research Program of Chongqing Municipal Education Commission
KJQN201902805/Scientific and Technological Research Program of Chongqing Municipal Education Commission
ygz2018103/Scientific and Technological Research Program of Chongqing Medical and Pharmaceutical College
YGZ2019402/Innovative Research Groups of Chongqing Medical and Pharmaceutical College
cstc2019jcyjbshX0128/postdoctoral Science Fund Project of Chongqing Natural Science Foundation
81901922/National Natural Science Foundation of China
Figures
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