IMR Press / JIN / Volume 22 / Issue 1 / DOI: 10.31083/j.jin2201022
Open Access Original Research
Molecular Characterization and Function of the Nogo-66 Receptor (NgR1) Gene in the Chinese Tree Shrew
Caixia Lu1,2,†Xiuying Kui1,2,†Xiaofei Li1,2,†Wenguang Wang1,2Xiaomei Sun1,2Na Li1,2Pinfen Tong1,2Jiejie Dai1,2,*
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1 Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, 650118 Kunming, Yunnan, China
2 Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, 650118 Kunming, Yunnan, China
*Correspondence: (Jiejie Dai)
These authors contributed equally.
Academic Editors: Rafael Franco and Jolanta Dorszewska
J. Integr. Neurosci. 2023, 22(1), 22;
Submitted: 25 April 2022 | Revised: 4 June 2022 | Accepted: 24 June 2022 | Published: 17 January 2023
Copyright: © 2023 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: Nogo-66 receptor (NgR1) is a glycosylphosphatidylinositol-linked cell surface receptor with high affinity for Nogo-66. The binding of Nogo-66 to NgR1 plays a key role in inhibiting neurite growth, limiting synaptic plasticity and mediating Mammalian Reovirus (MRV) infection. The Chinese tree shrew (Tupaia belangeri chinensis) is, a new and valuable experimental animal that is widely used in biomedical research. Although susceptible to MRV, little is known about tree shrew NgR1 and its role in MRV infection. Methods: In this study, we cloned NgR1 form the Chinese tree shrew by RACE technology and analyzed its characteristics, spatial structure and its tissue expression. We also examined the expression pattern of NgR1 in the response of tree shrew primary nerve cells (tNC) to MRV1/TS/2011 infection. Results: Tree shrew NgR1 was found to have a closer relationship to human NgR1 (90.34%) than to mouse NgR1. Similar to the protein structure of human NgR1, the tree shrew NgR1 has the same leucine-rich repeat (LRR) domain structure that is capped by C-terminal and N-terminal cysteine-rich modules. The tree shrew NgR1 mRNAs were predominantly detected in the central nervous system (CNS), and tree shrew NgR1 can mediate infection by MRV1/TS/2011. Conclusions: Taken together, these results help to elucidate the function of NgR1 and provide a basis for using the tree shrew as an animal model for studies of the nervous system and infectious diseases.

Nogo-66 receptor
tree shrew
Mammalian Reovirus
phylogenetic analysis
molecular characterization
D-2018026/Yunnan health training project of high-level talents
2018HB071/Yunnan science and technology talent and platform program
2019-1-R-24483/Kunming Science and technology innovation team
U1702282/National Natural Science Foundation of China
2017DG008/Yunnan province Key Laboratory project
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