Academic Editor: Rafael Franco
Background: To explore the mechanism of endocannabinoid cannabinoid
receptor 1 (CB1) receptor pathway that regulates synaptic plasticity in the
dorsal horn of the spinal cord of rats with neuropathic pain at different ages.
Methods: Neonatal, juvenile, and adult male sprague dawley (SD) rats
were divided into the spinal nerve preservation injury (SNI), SNI + Anandamide
(AEA), SNI + D-AP5, SNI + CNQX, SNI + D-AP5 + AEA, SNI + CNQX + AEA, sham SNI,
sham SNI + AEA, sham SNI + D-AP5, sham SNI + CNQX, sham SNI + D-AP5 + AEA, and
sham SNI + CNQX + AEA groups, respectively. Paw withdrawal threshold (PWT) and
long-term potentiation (LTP) of the spinal dorsal horn PS (field potential) were
assessed to judge the spinal cord’s functional state. Immunohistochemical
staining and Western blot were conducted to detect CB1 protein levels in the
spinal dorsal horn. Results: The LTP response in the spinal cord was
alleviated in the SNI + AEA group. After treatment with the N-methyl-D-aspartate
(NMDA) receptor blocker D-AP5, the LTP of neonatal A nerve was relieved further.
After treatment with the