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- Academic Editor
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†These authors contributed equally.
Background: Adipose tissue-derived stem cells (ADSCs), a type
of mesenchymal stem cell, have been used extensively in clinical trials for the
treatment of multiple conditions, including sepsis. However, increasing evidence
indicates that ADSCs vanish from tissues within days of administration.
Consequently, it would be desirable to establish the mechanisms underlying the
fate of ADSCs following transplantation. Methods: In this study, sepsis
serum from mouse models was used to mimic microenvironmental effects. Healthy
donor-derived human ADSCs were cultured in vitro in the presence of
mouse serum from normal or lipopolysaccharide (LPS)-induced sepsis models for the purposes of
discriminant analysis. The effects of sepsis serum on ADSC surface markers and
cell differentiation were analyzed by flow cytometry, and the proliferation of
ADSCs was assessed using a Cell Counting Kit-8 (CCK-8) assay. Quantitative real-time PCR (qRT-PCR) was applied to assess the degree
of ADSC differentiation. The effects of sepsis serum on the cytokine release and
migration of ADSCs were determined based on ELISA and Transwell assays,
respectively, and ADSC senescence was assessed by