IMR Press / FBL / Volume 12 / Issue 7 / DOI: 10.2741/2262

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Bacterial DNA microarrays for clinical microbiology: the early logarithmic phase
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1 Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, 1900 North 12th street, 19122 Philadelphia, PA, USA
2 Department of Molecular Biology, Laboratory of Molecular Microbiology and Biotechnology, University of Siena, Viale Bracci 53100 Siena, Italy
3 Department of Human Pathology and Oncology, University of Siena, Viale Bracci 53100 Siena, Italy
Academic Editor:Antonio Giordano
Front. Biosci. (Landmark Ed) 2007, 12(7), 2658–2669; https://doi.org/10.2741/2262
Published: 1 January 2007
(This article belongs to the Special Issue Gene targets for modulating cell growth)
Abstract

In this era of coexistence of high-throughput sequencing technologies and serious difficulties in the management of both common and novel infectious syndromes, new techniques which improve the study of micro-organisms is timely. In bacteriology, the most important subjects are bacterial pathogenicity, discovery of the genomic complexity of bacteria, and the epidemiology of antimicrobial resistance traits. From the clinical point of view, genetic testing is flanking phenotypic testing for the assessment of new, difficult to test antibiotic resistance traits, and for correlations with the microbial behaviour in vivo. The demand for faster, comprehensive and highly parallel microbial diagnostics is also cogent even at the basic laboratory level, where the ultimate objective is saving lives. In this setting, DNA microarrays offer a pivotal contribution by allowing performance of hybridization experiments in highly parallel formats, with an increasing reliability. Not only they are useful in deciphering host and microbial pathophysiology, they can also make the difference in the management of prognostic and therapeutic aspects of many diseases. Here, we provide an overview of the current use and the potential of DNA microarrays in clinical bacteriology, and several applications and technical solutions are discussed.

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