Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.
Mutation of siRNA results in thermodynamically unstable duplex which influences knockdown of dmrt1 by RNA interference
MicroRNAs (miRNAs) constitute a growing class of non-coding RNAs that are thought to regulate gene expression by translational repression and mRNA degradation. We report here that short interfering RNA (siRNA) mutation significantly changed kinetics of the folding and unfolding of secondary structures and decreased Tm value of the duplex melting. The mutant duplex was more unstable thermodynamically than the normal structure. Furthermore negative effects of the mutation on RNA interference (RNAi) was observed in both mouse dmrt1 transfected COS-7 and Sertoli cells in which endogenous dmrt1 was expressed. However, interference efficiency of mutational and normal duplex in Sertoli cells was not significant in comparison with those in dmrt1 transfected COS-7 cells, suggesting complex regulatory mechanisms in RNAi in endogenous dmrt1 expression. Abundance of intronic miRNA structures observed in the dmrt1 gene may also contribute to the precise regulation cascade in the dmrt1 expression. These findings help in further understanding RNA silence in vertebrate development and drug design for target gene.