IMR Press / FBE / Volume 5 / Issue 3 / DOI: 10.2741/E677

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Electroporation promotes HtrA1 uptake and in a mouse model of mesothelioma

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1 SAFU Department, Regina Elena Cancer Institute, Rome, Italy
2 Gene Expression and Human Molecular Genetics Laboratory, Institute of Genetics and Biophysics, CNR, Naples, Italy
3 Department of Oncology, Campus Biomedico University, Rome, Italy
4 Department of Biochemistry and Pathology, Second University of Naples, Naples, Italy
Academic Editor:Antonio Giordano
Front. Biosci. (Elite Ed) 2013, 5(3), 974–981; https://doi.org/10.2741/E677
Published: 1 June 2013
(This article belongs to the Special Issue Gene targets for modulating cell growth)
Abstract

There is not a consensus on the best therapeutic approach to mesothelioma and the prognosis is still dismal. We have recently demonstrated that HtrA1 is a potential therapeutic target in mesothelioma cells. In this manuscript we describe that electroporation in a mouse mesothelioma xenograft was able to facilitate the expression of exogenous HtrA1 injected intra-lesionally in the tumors and to increase the penetration in the neoplastic cells of cisplatin given intra-peritoneally. Indeed, HtrA1 over-expression caused a significant slowing down of tumor growth; moreover, cisplatin efficacy in reducing tumor mass was amplified by electroporation and this phenomenon was even more significant when combining the electroporation of cisplatin and HtrA1. Considering that a substantial number of mesothelioma patients develop early local recurrence, even with radical resection combined with aggressive chemo- and radiotherapy, this multi-modality approach could be very effective in improving local tumor control after surgery. The identification of effective combination coupled with the development of novel equipments and electrodes will be instrumental in planning the translation of these results to humans as per correct laboratory-clinical interface.

Keywords
Electrochemotherapy
HtrA1
CDDP
Biphasic Pulses
Mesothelioma
Xenograft
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