IMR Press / FBE / Volume 3 / Issue 2 / DOI: 10.2741/E260

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
HIV-1 integration site preferences in pluripotent cells
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1 Division of Infectious Diseases - Center for Human Virology, Thomas Jefferson University, Philadelphia
2 Center for Stem Cell Biology and Regenerative Medicine, Thomas Jefferson University, Philadelphia
3 Kimmel Cancer Center, Immunology Program, Thomas Jefferson University, Philadelphia
Academic Editor:Rene Daniel
Front. Biosci. (Elite Ed) 2011, 3(2), 453–462; https://doi.org/10.2741/E260
Published: 1 January 2011
(This article belongs to the Special Issue Targeted integration and gene therapy)
Abstract

HIV-1-based vectors are widely used in gene therapy. In somatic cells, these vectors mainly integrate within genes. However, no distinct integration site preferences have been observed with regard to large chromosomal regions. The recent emergence of induced pluripotent stem (iPS) cells, similar to embryonic stem (ES) cells, has raised questions about where integration occurs in these cells. In this work we investigated the integration site preferences of HIV-1-based vectors in a pluripotent, ES-like cell line. We show that approximately 30% of the integrations occur in the vicinity of telomeres. We have analyzed integration sites in various somatic cells, as reported by us and other groups, and observed that this integration pattern is unique to the analyzed pluripotent cell line. We conclude that pluripotent cells may contain distinct cellular cofactors that participate in integration targeting and that are not present in somatic cells.

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