IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E230

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

KHYG-1 and NK-92 represent different subtypes of LFA-1-mediated NK cell adhesiveness
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1 Blood Services Group, Health Sciences Authority, 11 Outram Road, Singapore,169078
2 School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551
3 Department of Haematology, St. George's Hospital and Medical School, Blackshaw Road, London, SW17 0PE, UK

*Author to whom correspondence should be addressed.


Front. Biosci. (Elite Ed) 2011, 3(1), 166–178;
Published: 1 January 2011

Novel cancer cellular therapy approaches involving long-term ex vivo IL-2 stimulated highly cytotoxic natural killer (NK) cells are emerging. However, adhesion properties of such NK cells are not very well understood. Herein, we describe the novel observation of permanently activated alphaLbeta2 integrin leukocyte function-associated antigen (LFA)-1 adhesion receptor in long-term IL-2 activated NK cells and the permanent NK cell lines KHYG-1 and NK-92. We show that such cytokine activated NK effectors constitutively adhered to the LFA-1-ligand ICAM-1, whereas binding to the lower affinity ligand ICAM-3 required additional exogenous activating conditions. The results demonstrate an extended conformation and an intermediate affinity state for the LFA-1 population expressed by the NK cells. Interestingly, adhesion to ICAM-1 or K562 induced pronounced cell spreading in KHYG-1, but not in NK-92, and partially in long-term IL-2 stimulated primary NK cells. It is conceivable that such differential adhesion characteristics may impact motility potential of such NK effectors with relevance to clinical tumor targeting. KHYG-1 could be a useful model in planning future targeted therapeutic approaches involving NK effectors with augmented functions.

Adoptive Immunotherapy
Natural killer (NK) cell
Lymphokine Activated Killer
LAK cells
leukocyte function-associated antigen
Intercellular Adhesion Molecule
cell spreading
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