IMR Press / JIN / Volume 18 / Issue 3 / DOI: 10.31083/j.jin.2019.03.174
Open Access Original Research
Dl-3-n-butylphthalide inhibits phenytoin-induced neuronal apoptosis in rat hippocampus and cerebellum
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1 Department of Neurology, Second Hospital of Hebei Medical University, 215 Hepingxi Road, Shijiazhuang 050000, Hebei, P. R. China
2 Department of Neurology, Affiliated Hospital of Hebei University, 212 East Yuhua Road, Baoding 071000, Hebei, P. R. China
3 Department of Neurology, People's Hospital in Shijiazhuang, 36 Fanxi Road, Shijiazhuang 050000, Hebei, P. R. China
*Correspondence: (Xiaopeng Wang)
These authors contributed equally.
J. Integr. Neurosci. 2019, 18(3), 277–283;
Submitted: 10 June 2019 | Accepted: 3 September 2019 | Published: 30 September 2019
Copyright: © 2019 Chen et al. Published by IMR press.
This is an open access article under the CC BY 4.0 license

Rats were divided into six groups: sham/control, Dl-3-n-butylphthalide, P1 (low phenytoin, 100 mg/kg), P2 (high phenytoin, 200 mg/kg), NP1 (Dl-3-n-butylphthalide 80 mg/kg, phenytoin 100 mg/kg), NP2 (Dl-3-n-butylphthalide 80 mg/kg, phenytoin 200 mg/kg). Hematoxylin/eosin and Nissl staining showed that, compared to the sham/control group, the Dl-3-n-butylphthalide group had no obvious hippocampal and cerebellar neuron loss, but there was a significant neuron loss in the P1 and P2 groups (P < 0.05), which was more obvious in the P2 group (P < 0.05). The positive expression of Bax and Bcl-2 proteins in hippocampal and cerebellar neurons was not significantly different between sham and Dl-3-n-butylphthalide groups; however, compared to sham, Bax expression was significantly increased and Bcl-2 was significantly decreased in the hippocampal and cerebellar neurons of rats in both P1 and P2 groups (P < 0.05), being more obvious in the P2 group (P < 0.05). Furthermore, the administration of Dl-3-n-butylphthalide attenuated the deleterious effects of phenytoin (P < 0.05). Our results indicate that phenytoin causes apoptosis of hippocampal and cerebellar neurons in rats in a dose-dependent manner, with the effect of a higher dose being more obvious, whereas, Dl-3-n-butylphthalide inhibits the phenytoin-induced apoptosis of neurons and has a neuroprotective role.

cerebellar neurons
hippocampal neurons
Bax protein
Bcl-2 protein
Figure 1.
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