IMR Press / FBS / Volume 9 / Issue 1 / DOI: 10.2741/S481

Frontiers in Bioscience-Scholar (FBS) is published by IMR Press from Volume 13 Issue 1 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


3D culture of mouse gastric stem cells using porous microcarriers

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1 Mechanical Engineering Department, College of Engineering, United Arab Emirates University, P.O. Box 15551, Al-Ain, UAE
2 School of Chemical and Bioprocess Engineering, University College Dublin, Dublin 4, Ireland
3 Department of Anatomy, College of Medicine, United Arab Emirates University, P.O. Box 17666, Al-Ain, UAE

*Author to whom correspondence should be addressed.


Front. Biosci. (Schol Ed) 2017, 9(1), 172–179;
Published: 1 January 2017

The lining epithelium of the stomach includes multipotent stem cells which undergo proliferation and migration-associated differentiation. These cells give rise to multiple cell lineages that produce mucus, acid, pepsinogen and various hormones/peptides. A 3D culture for stem cells would facilitate identification of the factors that control proliferation and/or differentiation programs. Here, we report on the use of disk-like ImmobaSil HD silicone-rubber matrix based microcarriers that are permeable to oxygen and reduce the creation of toxic environment within the center of the microcarrierd for culturing the mouse gastric stem (mGS) cells. We define several parameters that affect the initial cell attachment such as size of cell inoculum, serum concentration, mode and speed of agitation. We show that although such a microcarrier allows for attachment and growth of gastric stem cells, it does not lend itself and does not support the functional differentiation of such cells.

Stem Cells
Gastric Epithelium
Cell Culture
3D Culture
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