IMR Press / FBL / Volume 9 / Issue 3 / DOI: 10.2741/1315

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
DNA replication licensing
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1 Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, 812-8582, Japan
2 Department of Gen. Biology, School of Medicine, University of Patras, 26500 Rio, Patras, Greece
Front. Biosci. (Landmark Ed) 2004, 9(3), 2115–2132; https://doi.org/10.2741/1315
Published: 1 September 2004
Abstract

The DNA replication licensing system ensures that chromosomal DNA is replicated precisely once before cell division occurs. A DNA helicase must be loaded on origin DNA for replication to initiate. Considerable evidence suggests that the MCM complex acts as a replicative helicase in eukaryotes. When the MCM complex is loaded on the chromatin, the replication origin is formally defined as being licensed for replication. Licensing takes place several hours before origins are activated to undergo replication in S-phase. Genetic and biochemical studies show that the licensing process is well conserved in eukaryotes. Cyclin Dependent Kinases (CDKs), the master regulators of the cell cycle, coordinate the initiation of the two key cell cycle events, replication of DNA and its segregation at mitosis. Eukaryotes have developed complex regulatory mechanisms to ensure that origin licensing is coordinated with these events so that genome integrity is preserved during successive cell divisions.

Keywords
DNA replication
licensing
ORC
Cdc6
Cdt1
MCM
CDK
Review
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