IMR Press / FBL / Volume 8 / Issue 6 / DOI: 10.2741/1160

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

TNF-alpha and cortisone impair peptide chain initiation by altering the availability of initiation factor EIF-4E

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1 Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32114, USA
Front. Biosci. (Landmark Ed) 2003, 8(6), 1051–1055; https://doi.org/10.2741/1160
Published: 1 September 2003
Abstract

The effect of chronic tumor necrosis factor-α (TNF-α) and cortisone treatment on the concentration of translational initiation factor eIF-4E in rat skeletal muscle was evaluated. Crude muscle extracts from control and experimental groups (TNF-α s.c. @ 50 µg/kg body wt each day for 5 days or cortisone s.c. @ 100 mg/kg body wt for 5 days) were used to purify eIF-4E by immunoprecipitation and polyacrylamide gel electrophoresis (PAGE) followed by Western blot analysis. Quantification of eIF-4E was done by densitometry. Both TNF-α and cortisone induced a marked decline in the concentration of eIF-4E in rat skeletal muscle. There was no difference in the ratio of phosphorylated to unphosphorylated eIF-4E after TNF-α treatment. These findings suggest that both TNF-α and cortisone inhibit peptide chain initiation in skeletal muscle cells by decreasing the expression of eIF-4E.

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