IMR Press / FBL / Volume 7 / Issue 1 / DOI: 10.2741/A734

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


Protein denitration/modification by Escherichia coli nitrate reductase and mammalian cytochrome P-450 reductase

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1 Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32114
Front. Biosci. (Landmark Ed) 2002, 7(1), 9–14;
Published: 1 February 2002

The incubation of peroxynitrite (PN)-pretreated histone III-S (NH) with Escherichia coli nitrate reductase (cytochrome, NADPH/GSH-independent) and that of NADPH-treated NH (NHNADPH) with liver cytochrome P-450 reductase (NADPH-dependent) resulted in decreased 3-nitrotyrosine immunoreactivity found in Western blot analysis. Additionally, increased nitrate was noted as an end product of these reactions. These findings imply that varied enzymatic denitration/modification of NO/PN-reacted protein, either with or without a reductant, may be important in regulating related signal transduction cascade(s) and relieving oxidative stress.

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