IMR Press / FBL / Volume 23 / Issue 10 / DOI: 10.2741/4676

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Differential effects of the PI3K/AKT pathway on antler stem cells for generation and regeneration of antlers in vitro

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1 Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China
2 State Key Laboratory for Molecular Biology of Special Economic Animals, Jilin, China
3 AgResearch Ltd, Ruakura Research Centre, Private bag 3123, Hamilton 3240, New Zealand
Front. Biosci. (Landmark Ed) 2018, 23(10), 1848–1863; https://doi.org/10.2741/4676
Published: 1 June 2018
(This article belongs to the Special Issue Deer antler: a unique model for full mammalian organ regeneration)
Abstract

Understanding the role of the PI3K/AKT pathway in regulating basic antler stem cell parameters and angiogenesis may provide an insight into the mechanisms underlying mammalian appendage development. The present study took multiple approaches in vitro to investigate the effects of the PI3K/AKT pathway on antler stem cells. By addition of LY294002, proliferation rate of the antlerogenic periosteum (AP) cells was decreased significantly (p<0.01), while the proliferation rate of the pedicle periosteum (PP) cells decreased to a lesser extent; the cytoskeleton of the AP cells was essentially collapsed; and the PP cells significantly shrunken. By addition of LY294002 or KU-0063794, formation of networking tubular structures from HUVECs in the AP or PP cell conditioned medium was significantly inhibited; whereas, expression level of VEGF-B mRNA in the AP or PP cells was decreased by the former, and increased by the latter significantly. Therefore, the results suggest that the PI3K/AKT pathway is involved in proliferation and differentiation of the AP and the PP cells, and plays a more important role in the former than in the latter.

Keywords
PI3K/AKT pathway
Antler Stem Cell
Antler generation
Angiogenesis
VEGF-B
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