IMR Press / FBL / Volume 19 / Issue 5 / DOI: 10.2741/4239

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
The change of break modulus drives human fibroblast differentiation in 3D collagen gels
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1 Department of Burn and Plastic Surgery, Ruijin Hospital, Shanghai Burn Institute, Shanghai Jiaotong University School of Medicine, Shanghai, China
2 Rehabilitation Center of Plastic and Burn, First Affiliated Hospital of GuangXi Medical University, Nanning, China
Academic Editor:Fei He
Front. Biosci. (Landmark Ed) 2014, 19(5), 727–733;
Published: 1 January 2014
(This article belongs to the Special Issue Cellular immunology and stem cell biology)

Extracellular matrix is one of the key environmental factors influencing cell survival and provides signals for cell morphological change, migration, proliferation and differentiation. However, the mechanism through which denatured collagen modulates the biological properties of fibroblasts, is unclear. We investigated the regulation of human fibroblast differentiation in vitro grown in collagen gels with different properties. The break modulus of collagen with denatured collagen and half-load normal collagen was reduced compared with that of normal collagen gel. Fibroblasts cultured in denatured collagen gels showed increased expression of matrix metalloproteinase9 ( MMP-9), tissue inhibitor of metalloproteinase 2(TIMP2), α-smooth muscle actin (α-SMA), osteoblast cadherin, phosphorylated Myosin phosphatase target subunit1 (p-MYPT1), connective tissue growth factor, type I collagen, type III collagen, α-smooth muscle actin messenger RNA, RhoA, rho-associated protein kinase, and transforming growth factor β receptors 1 and 2 compared with that in cells cultured in normal collagen gel. But there was no significant difference regarding expression level between denatured collagen gel and half-load normal collagen gel .These findings suggest that the change in break modulus caused by decreasing normal collagen concentration may be the key factor inducing fibroblast differentiation.

Three-dimensional collagen gel
Denatured Collagen
Fibroblast Cells
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