IMR Press / FBL / Volume 18 / Issue 4 / DOI: 10.2741/4178

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


Use of the Ussing chamber technique to study nutrient transport by epithelial tissues

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1 Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center for Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center for Animal and Poultry Science, Key Laboratory of Agroecology in Subtropical Region, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central China, Ministry of Agriculture, Changsha 410125, Hunan, Peoples R China
2 State Key Laboratory of Food Science and Technology, College of Life Science and Food Engineering, Nanchang University, Nanchang, Jiangxi 330047, China
3 State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193, China
4 Department of Animal Science, Texas A and M University, College Station, TX 77843-2471, USA
Front. Biosci. (Landmark Ed) 2013, 18(4), 1266–1274;
Published: 1 June 2013

The Ussing chamber provides a physiologically relevant system for measuring the transport of ions, nutrients, and drugs across various epithelial tissues. This article outlines the design, structure, principle, and operation of the Ussing chamber, its application in the field of gastrointestinal barrier function and nutrient transport research, as well as its advantages and limitations. This review serves as a practical guide for investigators who are new to the Ussing chamber and should help researchers better understand this valuable method for measuring the transport of electrolytes, organic nutrients, water, and drugs across the small intestine, placenta, and other epithelial tissues.

Ussing chamber
Nutrient transport
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