IMR Press / FBL / Volume 17 / Issue 5 / DOI: 10.2741/4020

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Processing-independent analysis of peptide hormones and prohormones in plasma
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1 Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark
2 Department of Small Animal Clinical Sciences, University of Copenhagen, Denmark
3 Department of Gastrointestinal Surgery, Rigshospitalet, Copenhagen, Denmark

Academic Editor: Darja Kanduc

Front. Biosci. (Landmark Ed) 2012, 17(5), 1804–1815; https://doi.org/10.2741/4020
Published: 1 January 2012
(This article belongs to the Special Issue Peptides: from basic research to clinical applications)
Abstract

Peptide hormones are post-translationally matured before they reach a structure in which they can fulfill their biological functions. The prohormone processing may encompass a variety of endoproteolytic cleavages, N- and C-terminal trimmings, and amino acid derivatizations. The same prohormone can be variably processed in different cell types and, in addition, diseased cells often change the processing of a given precursor. The translational process is often either increased or decreased in diseased cells, which renders the ensuing modifications of the prohormone incomplete. Consequently, a variable mixture of precursors and processing-intermediates accumulates in plasma. In order to exploit disturbed posttranslational processing for diagnostic use and at the same time provide an accurate measure of the translational product, a simple analytical principle named "processing-independent analysis" (PIA) was designed. PIA-methods quantitate the total mRNA product irrespective of the degree of processing. PIA-methods have now been developed for a number of prohormones and proteins, and their diagnostic potential appears promising in diagnosis of cardiovascular disease and in several malignancies.

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