IMR Press / FBL / Volume 13 / Issue 11 / DOI: 10.2741/2987

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Iron oxide - loaded liposomes for MR imaging

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1 Department of Anatomy, Christian-Albrechts-University zu Kiel
2 Department of Medical Physics, University Clinic Schleswig-Holstein, Campus Kiel, Germany
3 Section of Neuroradiology, University Clinic Schleswig-Holstein, Campus Kiel, Germany

*Author to whom correspondence should be addressed.

Academic Editor: Fabian Kiessling

Front. Biosci. (Landmark Ed) 2008, 13(11), 4002–4008; https://doi.org/10.2741/2987
Published: 1 May 2008
(This article belongs to the Special Issue Molecular imaging in cancer)
Abstract

In this study a liposome cell labeling system was developed for non - target - specific labeling of glioma cells with superparamagnetic iron oxide nanoparticles for magnetic resonance imaging (MRI). A high non - target - specific uptake is ideal for in vitro labeling of cells and subsequently for cell tracking and visualization of phagocytic cells in vivo. The preparation of iron oxide - loaded liposomes was optimized and the biological properties of the liposomes were investigated. Cytotoxicity and cell viability were examined and showed limited cytotoxic effects. Non - target - specific labeling of glioma cells in vitro for subsequent specific labeling of molecules for MR imaging was tested by T2*-weighted MRI at 3T. The glioma cells showed a strong initial uptake of the iron oxide liposomes and the uptake was not saturable within 24 h exposure. The uptake of liposomes was superior to non - coated magnetite nanoparticles. Using PEG - ylated liposomes, the non - specific uptake could be decreased fundamentally (86% lower) in comparison to conventional liposomes. Furthermore, the ability of liposomes as contrast agents for MR imaging was investigated. Cells labeled with iron oxide nanoparticles by treatment with liposomes showed a negative contrast in MRI and consequently successful cellular labeling. Thus, iron oxide - loaded liposomes are well suited for non - target - specific cell labeling for MR imaging.

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