IMR Press / JIN / Volume 19 / Issue 4 / DOI: 10.31083/j.jin.2020.04.203
Open Access Original Research
Bioinformatic analysis of a microRNA regulatory network in Huntington’s disease
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1 Department of Neurology, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, 110004, P. R. China
*Correspondence: (Shu-Yan Cong)
J. Integr. Neurosci. 2020, 19(4), 641–650;
Submitted: 25 June 2020 | Revised: 21 October 2020 | Accepted: 29 October 2020 | Published: 30 December 2020
Copyright: © 2020 Wang et al. Published by IMR Press.
This is an open access article under the CC BY 4.0 license (

Huntington’s disease is an autosomal dominant hereditary neurodegenerative disease characterized by progressive dystonia, chorea and cognitive or psychiatric disturbances. The leading cause is the Huntington gene mutation on the patient’s chromosome 4 that produces a mutated protein. Recently, attention has focused on the relationship between microRNAs and Huntington’s disease’s pathogenesis. In Huntington’s disease, microRNAs can interact with various transcription factors; dysregulated microRNAs may be associated with the Cytosine deoxynucleotide-Adenine ribonucleotides-Guanine ribonucleotide length and Huntington’s disease’s progression and severity. This study explores the role of microRNAs in the pathogenesis of Huntington’s disease through bioinformatics analysis. By analyzing data from the Gene Expression Omnibus database, we identified a total of 9 differentially expressed microRNA. Subsequently, target genes and long non-coding RNAs were predicted, and a comprehensive regulatory network centered on microRNA was constructed. The microRNA integrated regulatory network, Homo sapiens (hsa)-miR-144-3p, interacted with the largest number of long non-coding RNAs, including X-inactive specific transcript and taurine upregulated gene 1. The miRNAs, hsa-miR-10b-5p and hsa-miR-196a-5p, regulated most of the target genes, including class I homeobox and brain-derived neurotrophic factor genes. Additionally, 59 Gene Ontology terms and eight enrichment pathways were identified by analyzing the target genes of hsa-miR-196a-5p and hsa-microRNA-10b-5p. In conclusion, hsa-miR-10b-5p and hsa-miR-196a-5p were significantly and differentially expressed in Huntington’s disease, the long non-coding RNAs X-inactive specific transcript, taurine upregulated gene 1, and target genes such as homeobox or brain-derived neurotrophic factor may play critical roles in the pathogenesis of Huntington’s disease.

Huntington's disease
long non-coding RNAs
target gene
regulatory network
81371271/National Natural Science Foundation of China
Fig. 1.
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