IMR Press / JIN / Volume 21 / Issue 3 / DOI: 10.31083/j.jin2103091
Open Access Original Research
Evaluation methods of a rat sciatic nerve crush injury model
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1 Department of Tuina, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 200437 Shanghai, China
2 Acupuncture and Tuina College, Shanghai University of Traditional Chinese Medicine, 201023 Shanghai, China
*Correspondence: (Jun-Tao Yan); (Yun An)
These authors contributed equally.
Academic Editor: Robert Friedman
J. Integr. Neurosci. 2022, 21(3), 091;
Submitted: 8 September 2021 | Revised: 30 December 2021 | Accepted: 7 January 2022 | Published: 11 May 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: The rat sciatic nerve crush injury model is one of the most commonly used models to research peripheral nerve injury (PNI), however, the evaluation of the model preparation lacks exact standards. This study aimed to investigate accurately assessment methods for research concerning the rat sciatic nerve crush injury. Methods: The sciatic nerve crush injury model of was performed using the FST toothless forceps. The corresponding locations and pressures of different ratchet strengths were assessed by using CMAP, behavioral, and morphological methods. Results: In each group of PNI, motor and sensory functions were gradually restricted on the injured side of rats as the applied pressure increased. CMAP was more sensitive to nerve injury arising out of the force values obtained from the forceps. Conclusions: As a sensitive indicator for PNI, the neuroelectrophysiological examination was more likely to reflect the morphological changes of injured nerves. These findings may provide a standardized approach to sciatic crush injury modelling.

Sciatic nerve crush injury
Fig. 1.
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