Background: Uterine myoma, which seriously threatens women’s physical and mental health and quality of life, is the most common benign pelvic tumor in gynecology. At present, laparoscopic myomectomy (LM) has become an effective and commonly surgical method in clinical practice. The aim of this study, using meta-analysis method, is to analyze the advantages and disadvantages of baseball suture comparing with traditional one, and provide a theoretical basis for the selection of surgical suture methods. Methods: CNKI, WanFang Data, VIP, PubMed, web of science and the Cochrane Library are searched to collect original data and cases. Two researchers independently screen the literature according to the inclusion and exclusion criteria, extract the data and cross check them. Then RevMan 5.4 and STATA 14 are used for statistical analysis. The difference is statistically significant (p 0.05). Results: Compared with the traditional suture method, the operation time of LM with baseball suture method is shorter, the amount of intraoperative bleeding is less, the time required for postoperative exhaust, first getting out of bed, hospitalization, and uterine incision suture is shorter, the incidence of needle eye bleeding is lower, the decline of average hemoglobin is lower, and the incidence of pelvic infection is lower, while the difference is statistically significant (p 0.05). The incidence of postoperative morbidity is lower, while the difference is not statistically significant (p > 0.05). Conclusions: Baseball suture has progressiveness and important value in LM surgical suture. The hemostasis and healing effect is ideal, which can enable patients to recover more quickly, reduce the pain and economic burden caused by the operation, and also enable surgeons to complete the operation more conveniently and efficiently. The protocol of the review was registered on the PROSPERO platform (https://www.crd.york.ac.uk/PROSPERO/) with the registration number CRD42022370262.