Aim: Domestic violence is a social problem with increasing dimensions worldwide. The various forms of abuse and especially violence during pregnancy have not been sufficiently studied by the Greek scientific community. The aim of this study was to translate, culturally adapt and validate a special research tool that can be used by health professionals as a diagnostic tool for violence during pregnancy. Methods: The Abuse Assessment Screen (AAS) questionnaire was chosen as a screening tool. The questionnaire was translated into Greek in accordance with the procedure suggested by the ‘Trust Scientific Advisory Committee’, followed by the cultural adaptation of the questionnaire to the Greek reality. Results: Specific psychometric tests were used for the validation of the questionnaire in order to assess the questionnaire’s reliability and validity, and a factor analysis was also carried out. The internal consistency for all the parties who were questioned (n = 262), as expressed by Cronbach’s alpha coefficient for the AAS, was 0.806 which is quite satisfactory and the results of our study suggest that the Greek translation of the AAS has a high correlation index compared to relevant international studies. Conclusions: The AAS questionnaire in the Greek version seems to be a reliable and valid tool for the diagnosis of violence during pregnancy.