IMR Press / CEOG / Volume 51 / Issue 2 / DOI: 10.31083/j.ceog5102032
Open Access Original Research
Factors Associated with the Occurrence of 0PN Fertilization in in Vitro Fertilization and its Effects on Embryo Development and Reproductive Outcomes
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1 Reproductive Medical Center, Liaocheng People's Hospital, 252000 Liaocheng, Shandong, China
*Correspondence: (Jing Du)
These authors contributed equally.
Clin. Exp. Obstet. Gynecol. 2024, 51(2), 32;
Submitted: 25 September 2023 | Revised: 10 November 2023 | Accepted: 22 November 2023 | Published: 4 February 2024
(This article belongs to the Special Issue In-Vitro Fertilization (IVF))
Copyright: © 2024 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.

Background: To investigate factors associated with the occurrence of nonpronuclear (0PN) fertilization and to determine its effects on embryo development and reproductive outcomes in conventional in vitro fertilization (cIVF). Methods: This retrospective cohort study included 1116 IVF cycles and the corresponding 535 fresh transfer cycles performed during 2016–2022. Patients were divided into 0PN (+) (n = 279) and 0PN (–) (n = 837) groups based on whether they had embryos with 0PN fertilization. A multiple logistic regression model was used to determine confounders that could affect 0PN fertilization. Additionally, embryo development and reproductive outcomes were compared between the 0PN (+) and 0PN (–) groups, as well as between the 0PN embryo (n = 563) and two pronuclear (2PN) embryo (n = 1976) groups. Results: The number of mature oocytes was significantly correlated with the occurrence of 0PN fertilization (odds ratio: 1.15; 95% confidence interval 1.04–1.27; p = 0.007). The top-quality embryo rate was significantly higher in the 0PN (+) group than in the 0PN (–) group (52.9% vs. 49.9%; p = 0.024). Clinical outcomes were similar between these groups in terms of clinical pregnancy rates (43.1% vs. 45.5%), implantation rates (35.0% vs. 34.1%), miscarriage rates (15.3% vs. 17.1%), and live birth rates (33.6% vs. 33.9%). The top-quality embryo rates (45.3% vs. 52.9%, p = 0.001) and blastulation rates (57.7% vs. 63.8%, p = 0.023) were significantly lower in 0PN embryos than in 2PN embryos. The clinical outcomes were also similar between these groups. Conclusions: The number of mature oocytes is significantly correlated with the occurrence of 0PN fertilization in cIVF. 0PN fertilization does not affect reproductive outcomes during fresh transfer cycles.

nonpronuclear fertilization
in vitro fertilization
embryo development
embryo transfer
2019-0888/TCM Science and Technology Development Plan Project of Shandong Province
2018ws424/Medical and Health Technology Development Plan Project of Shandong Province
Fig. 1.
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