IMR Press / CEOG / Volume 44 / Issue 4 / DOI: 10.12891/ceog3426.2017

Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 46 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
The effects of sildenafil and tadalafil on ischemia–reperfusion injury in rat ovarian torsion model
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1 Department of Obstetrics and Gynecology, Medical Faculty of Hitit University, Corum, Turkey
2 Department of Obstetrics and Gynecology, Zekai Tahir Burak Women’s Health Training and Research Hospital, Ankara, Turkey
3 Department of Physiology, Medical Faculty of Hacettepe University, Ankara, Turkey
4 Department of Pathology, Diskapi YB Training and Research Hospital, Ankara, Turkey
5 Department of Obstetrics and Gynecology, Medical Faculty of Duzce University, Duzce, Turkey
6 Department of Biochemistry, Diskapi YB Training and Research Hospital, Ankara, Turkey
7 Department of Endocrinology, Diskapi YB Training and Research Hospital, Ankara, Turkey
8 Pancreas Islet Research Center, Diskapi YB Training and Research Hospital, Ankara, Turkey
9 Department of Endocrinology and Metabolism, Kastamonu Medical Faculty of Hacettepe University, Ankara, Turkey
Clin. Exp. Obstet. Gynecol. 2017, 44(4), 535–539; https://doi.org/10.12891/ceog3426.2017
Published: 10 August 2017
Abstract
Objective: In cases of ovarian torsion (OT), reperfusion injury may further damage ovarian tissue. Tadalafil and sildenafil are phosphodiesterase-5 (PDE-5) inhibitors that are primarily used for treatment of erectile dysfunction and pulmonary hypertension. In this study, the authors investigated the effects of these agents in preventing ovarian ischemia-reperfusion injury in a rat OT model. Materials and Methods: A total of 48 young adult female Wistar Albino rats were randomized into six groups (n=8 in each group); group 1: controlsham operated, group 2: sildenafil one mg/kg, group 3: tadalafil one mg/kg, group 4: sildenafil one mg/kg + ischemia/reperfusion, (I/R) two hours of ischemia and two hours of reperfusion and a single dose of oral one mg/kg sildenafil one hour before I/R, group 5: I/R, and group 6: tadalafil one mg/kg + I/R two hours of ischemia and two hours of reperfusion and a single dose of oral one mg/kg tadalafil one hour before I/R. Histopathologic evaluation was performed and scored according to the degree of congestion, edema, and hemorrhage. Total histological score (THS), tissue protein carbonyl (PC), malondialdehyde (MDA) levels, and catalase (CAT) activity were also calculated. Results: Group 4, group 5, and group 6 had similar congestion, edema and THS (p > 0.05). Group 6 had significantly lower hemorrhage score, when compared with group 5 (p = 0.03). Mean PC, MDA levels, and CAT were similar between groups 4, 5, and 6 (p > 0.05). Conclusions: Tadalafil was associated with a decreased tissue hemorrhage score in I/R injury in rat ovarian torsion model.
Keywords
Reperfusion injury
Phosphodiesterase inhibitors
Ovary
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