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Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 47 Issue 1 (2020). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.
Original Research
Ultrastructural analysis of granulosa cells of IVF patients
L. Karakoc Sokmensuer1,2,*, F.F. Kaymaz2
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1
Hacettepe University, Faculty of Medicine, Department of Obstetrics and Gynecology, Ankara
2
Hacettepe University, Faculty of Medicine, Department of Histology & Embryology, Ankara (Turkey)
Clin. Exp. Obstet. Gynecol. 2015, 42(5), 629–633;
https://doi.org/10.12891/ceog1934.2015
Published: 10 October 2015
Abstract
Objective: To compare the percentage and ultrastructure of normal and abnormal granulosa cells and their effect on fertilization and pregnancy rate between gonadotropin releasing hormone (GnRH) agonist and antagonist treatment. Materials and Methods: In this study, granulosa cells obtained from 22 women undergoing in vitro fertilization (IVF) treatment due to unexplained infertility with either with GnRH agonist (n=11) or GnRH antagonist (n=11) were evaluated by light and electron microscopy. Results: GnRH agonist and antagonist therapy was found to have no effect in terms of abnormal granulosa cell percentage (0.0679 ± 0.08977 vs 0.0481 ± 0.05164; p > 0.05), fertilization [85 (45-90) vs 75 (64-93)] and pregnancy rate (37% vs 46%). Light microscopic observations showed similar features of normal cells of agonist and antagonist-treated cells. Ultrastructural evaluation also revealed that there was no difference between cells of two treatment groups. Conclusion: Both GnRH agonist and antagonist treatment for ovarian stimulation may have similar effects on granulosa cells at the morphological and ultrastructural level, as well as on fertilization and pregnancy rates.
Keywords
Granulosa cell
Ultrastructure
In vitro fertilization
Fertilization rate
Pregnancy rate