Objective: A live birth after transfer of vitrified-warmed blastocyst derived from intracytoplasmic sperm injection (ICSI) with frozenthawedsperm of a male cancer patient is described. Materials and Methods: A case report from a tertiary center for assisted reproductivetechnologies. The 35-year-old male patient had been diagnosed with testicular tumor nine years ago. He had unilateral orchiectomyoperation after the diagnosis. Four years after the first operation, he was diagnosed with another testicular tumor in the other testis. Headmitted to our center with the demand of sperm preservation before the second surgery. The sperm samples were cryopreserved andstored in liquid nitrogen until required. The patient had no chemotherapy or radiotherapy after the operations. After he completed hisoncologic follow up, ICSI was decided with his frozen samples. Although the couple failed to conceive with the fresh cycle, the remainingembryos were frozen and revealed a pregnancy in the subsequent frozen-thawed cycle. Results: A healthy female infant with a birthweight of 3,700 g was born by cesarean section at 38th weeks of the gestation. Conclusion: Giving detailed information about fertilitysavingmanagement in male patients is important in those who wish to bear children. However, both the patients and physicians shouldbe cautious that preservation should be performed before surgery and/or adjuvant therapy. In this respect, assited reproductive technology(ART) and related facilities yield chance of pregnancy in such population.