Purpose of investigation: To evaluate the efficacy of sperm pooling in the treatment of male infertility with the use of intrauterine tuboperitoneal insemination (IUTPI). Materials and Methods: A total of 169 cycles of IUTPI were performed in 69 couples with mild male factor infertility. Pooled semen samples were used in 115 cycles (Group A), whereas a single sample was used in 54 (Group B). The same mild ovarian stimulation protocols were used in all cycles. Results: The mean inseminate motile count (IMC), following sperm pooling was 6.63 x 106 in Group A and 3.74 x 106 in Group B (p = 0.0001) with a single semen sample. In total, 33 clinical pregnancies were achieved; 28 (24%) in Group A and five (9%) in Group B (p = 0.036). Conclusions: The results of this study indicate that sperm pooling may prove a useful technique in the treatment of mild male infertility when combined with IUTPI.