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Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 47 Issue 1 (2020). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.
Original Research
Molecular diagnosis of CMV infection in fetal aborted tissues in the region of Thrace
I. Grammatikopoulou1, M. Lambropoulou1, E. Chatzaki2, T.E. Deftereou1, V. Lambropoulou1, M. Simopoulou3, E. Papadopoulos1, G. Galazios4, Th. Dimitriou5, A. Petrou1, N. Papadopoulos1,*
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1
Laboratory of Histology-Embryology, Medical School, Democritus University of Thrace
2
Laboratory of Pharmacology, Medical School, Democritus University of Thrace
3
Laboratory of Physiology, Medical School, Democritus University of Thrace
4
Department of Obstetrics and Gynecology, Medical School, Democritus University of Thrace
5
Laboratory of Anatomy, Medical School, Democritus University of Thrace, Alexandroupolis (Greece)
Clin. Exp. Obstet. Gynecol. 2012, 39(1), 96–102;
Published: 10 March 2012
Abstract
Purpose: To detect the incidence of CMV infection in spontaneous abortion in Thrace. Methods: Genetic material from 143 fetuses aged from 11 to 39 weeks was examined. The material originated from various regions of Thrace. All fetuses and the respective placentas underwent routine histopathology. DNA was isolated from sections of paraffinized tissues. Detection of CMV in the DNA genomic samples was performed using a commercial PCR-based detection kit. Results: From the 143 fetuses that were examined, two were found to be CMV positive. Pathological findings related to inflammatory corruptions were observed in the placentas of 97 embryos, including the CMV infected ones. Conclusions: This study indicates CMV-DNA infection in 1.4% of aborted fetuses. CMV infection incidence in aborted fetuses is similar to this reported in other European regions. The molecular technique of PCR applied on paraffin-embedded biopsy material is proven to be an accurate, valid and fast method for investigating the CMV infection in aborted fetuses.
Keywords
Spontaneous abortion
Fetus
Cytomegalovirus - CMV
PCR