It has been established that nonsteroidal anti-inflammatory drugs (NSAIDs) have additional mechanisms of action, besides the inhibition of prostanoid production. We evaluated the ability of lornoxicam, a novel NSAID of the oxicam family, to interfere with the migration of human polymorphonuclear cells (PMN) in vitro. PMN were obtained from healthy donors. Chemotaxis was assessed by a Boyden microchemotaxis chamber and was stimulated with the bacterial chemoattractant formyl-met-leu-phe (fMLP, 0.1 μM), the chemokine Interleukin-8 (25 ng mL -1, 0.003 μM) or the neuropeptide substance P (0.1 μM). Lornoxicam was used at the concentrations of 0.01, 1.0 and 100 μM. This NSAID inhibited fMLP and Substance P chemotaxis starting from the concentration of 1.0 μM, whereas Interleukin-8-induced chemotaxis was significantly reduced also at 0.01 μM. As these concentrations can be easily reached in plasma and in the synovial fluid after the administration of this drug in vivo, present data suggest that the inhibition of neutrophil migration is involved in the anti-inflammatory action of lornoxicam.