†These authors contributed equally.
Academic Editor: Graham Pawelec
Introduction: Exploration of novel and effective antiplatelet
strategies for the secondary prevention of ischemic stroke is utmost. Some
platelet derived microparticles (PMVs) in convalescent stroke subjects were found
to be predictive for the next vascular event. Patients with high-on-treatment
platelet reactivity (HTPR) had a significantly higher risk for ischemic stroke.
Here, we aimed to explore associations among circulating microparticles and
responsivness to antiplatelet (clopidogrel) therapy. Methods: A total of
18 patients on clopidogrel therapy due to secondary stroke prevention were
rospectively recruited into this study. Twenty age-matched healthy subjects
served as controls. Flow cytometric measurements of microparicles (MVs) and data
analysis were performed on Beckman-Coulter FC-500 cytometer with CXP software.
Besides, platelet aggregometry data were revealed. Both measurements were
performed in whole blood and from the lower and upper blood fractions separated
after 1-hour gravity sedimentation by the analogy with erythrocyte sedimentation
rate. Results: The total number of circulating MVs, and particularly the
platelet derived CD42