†These authors contributed equally.
Academic Editor: Marcello Iriti
Background: Many drugs for anti-tumour have been developed,
nevertheless, seeking new anticancer drug is the focus of ongoing investigation.
Withanolides have been reported to possess potent antiproliferative activity.
Literature findings revealed that a diversity of withanolides were obtained
from Physalis peruviana, however, the antitumor activity of these
bioactive compounds is still unclear. Methods: The EtOAc fraction of
P. peruviana were decolorized on Middle Chromatogram Isolated (MCI) Gel
column, repeatedly subjected to column chromatography (CC) over sephadex LH-20,
preparative High Performance Liquid Chromatography (HPLC) and silica gel to
afford compounds. Their chemical structures of the new isolates were elucidated
through analyzing spectroscopic and HRESIMS data. All these obtained metabolites
were appraised for their potential antiproliferative activity against the human
breast cancer cell line MCF-7 by MTT assay, and in vitro antibacterial
activity of the isolated compounds (1–7) were evaluated
against E. coli, B. cereus and S. aureus.
Results: Four new withanolides, including one
withaphysalin-type withanolide (peruranolide A, 1), two
13,14-seco-withaphysalins (peruranolides B–C, 2–3), as well
as one normal withanolide (peruranolide D, 4), were purified and
separated from P. peruviana L.. Compound 5 was discovered to
exhibit potent cytotoxic effect with an IC