This study reports for the first time the antifungal activities of acetone, hexane, dichloromethane and methanol leaf extracts of four plant species (Acacia pennata, Anaphalis wightiana, Capparis pepiaria and Catunaregum spinosea) from Belgaum district of Karnataka state, India. The antifungal activities were determined against pathogens (Candida albicans, Kluyeromyces polysporous, Aspergillus niger, Aspergillus fumigatus) representing yeasts, moulds and non-thermal dimorphic fungi. MIC values were determined by checking the growth after 24 and 48 h to determine the antifungal activity against the tested pathogens. Highest antifungal activity was observed with methanolic extracts of Anaphalis wightiana against all the tested pathogens with the MIC values ranging from 0.02 to 0.06. Methanolic extracts of Acacia pennata, Anaphalis wightiana, Capparis pepiaria have very strong antifungal activity against tested pathogens particularly C. albicans and K. polysporus. The overall results provide promising baseline information for the potential use of the crude extract of leaf in the treatment of fungal infections. An attempt has been made to highlight the promising plant species for further investigation as leads for new drug development.