Background and Objective: Wogonin belongs to active flavonoids and has been reported with several biological and pharmacological potentials. Wogonin has been reported with anticancer activity along with other biological applications. The anticancer effects of wogonin are mediated via its mitochondrial apoptosis and G2/M cycle arrest. Therefore, wogonin was herein testified for inhibition of multiple myeloma (MM) growth involving mitochondrial apoptosis and G2/M cycle arrest. Materials and Methods: The MTT assay was employed to test the cellular viability of MM cells. Apoptosis estimations were performed with Annexin V/PI staining assay, AO/EB staining assay and western blotting assay. The cell cycle in MM cells was analysed through flow cytometry. Results: The wogonin treatment on multiple melanoma cells induced anti-viability effects in a dose-reliant fashion. Moreover, it was shown that the anti-viability properties of wogonin are facilitated by its ability to induce apoptosis. According to the study’s results, the wogonin treatment led to a dose-dependent cell cycle arrest in the G2/M phase, as verified by cell cycle monitoring. Conclusion: The wogonin-induced inhibition of MM growth involves mitochondrial apoptosis and G2/M cycle arrest. Therefore, it can prove a leading candidate in MM research and treatment provided further investigations.