Background and Objective: Diabetes mellitus is a devastating condition and an important risk factor for the delayed healing process, leading to chronic Diabetic Foot Ulcers (DFUs). L-glutamine showed its antidiabetic and wound healing potential against various experimental models. To evaluate the potential of L-glutamine against the STZ-induced excision wound model, which mimics the clinical characteristic of DFU. Materials and Methods: Wounds (2×5 mm) were created on the dorsal surface of the paw of diabetic Sprague Dawley rats and then they were treated with either vehicle or L-Glutamine (250, 500 and 1000 mg kg–1, p.o.) or insulin (10 IU mg kg–1, s.c.) for 21 days. A group of non-diabetic and normal wound control animals was maintained separately. Results: L-Glutamine (500 and 1000 mg kg–1) treatment effectively (p<0.05) accelerates wound healing revealed by an increased rate of wound contraction and decreased wound area compared to control group animals. Diabetes-induced elevated oxido-nitrosative stress (SOD, GSH, MDA and NO) and proinflammatory release (TNF-α and IL-1β) were markedly (p<0.05) attenuated by L-Glutamine. Down-regulation production of hydroxyproline and mRNA expressions of Nrf2 and Ang-1in wound tissues was effectively (p<0.05) increased by L-Glutamine. It also significantly attenuated (p<0.05) diabetes-induced alteration in wound architecture. Conclusion: L-glutamine improve the wound healing process via accelerated angiogenesis and inhibition of hyperglycemia, oxidative stress and release of inflammatory mediators in experimental diabetic foot ulcer.