IMR Press / FBE / Volume 3 / Issue 4 / DOI: 10.2741/E349

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Garcinol-induced apoptosis in prostate and pancreatic cancer cells is mediated by NF-κB signaling

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1 Department of Pathology, Wayne State University School of Medicine and Barbara Ann Karmanos Cancer Center, Detroit, MI 48201, USA
2 ISTRA, Abeda Inamdar College, Pune 411001, India

*Author to whom correspondence should be addressed.

Academic Editor: Mukesh Agarwal

Front. Biosci. (Elite Ed) 2011, 3(4), 1483–1492; https://doi.org/10.2741/E349
Published: 1 June 2011
(This article belongs to the Special Issue Novel strategies for detection, prevention and treatment of cancer)
Abstract

Garcinol, obtained from Garcinia indica, is a potent antioxidant. Its anticancer activity has been investigated; however, there is no published report on its action against prostate and pancreatic cancer cells. We have earlier reported its activity against breast cancer cells, and here we tested our hypothesis that garcinol could inhibit cell proliferation and induce apoptosis in prostate as well as pancreatic cancer cells. Using multiple techniques such as MTT, Histone-DNA ELISA, activated caspase assays, clonogenic assays and EMSA, we investigated the mechanism of apoptosis-inducing effect of garcinol in prostate (LNCaP, C4-2B and PC3) and pancreatic (BxPC-3) cancer cells. We found that garcinol inhibited cell growth of all the cell lines tested with a concomitant induction of apoptosis in a dose-dependent manner. Down-regulation of NF-κB signaling pathway appears to be the mechanism of apoptosis-induction because garcinol inhibited constitutive levels of NF-κB activity, which was consistent with down-regulation of NF-κB-regulated genes. A significant decrease in the colony forming ability of all the cell lines was also observed, suggesting the possible application of this compound against metastatic disease. In summary, our results provide pre-clinical evidence to support the use of garcinol against human prostate and pancreatic cancer, thus meriting its further investigation as a potential chemo-preventive and/or therapeutic agent.

Keywords
Garcinol
Prostate cancer
Pancreatic cancer
NF-κB
Apoptosis
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