† These authors contributed equally.
Balanced skin microbiota is crucial for maintaining healthy normal skin
function; however, disruption of the balance in skin microbiota is linked with
skin diseases such as atopic dermatitis, acne vulgaris, dandruff, and
candidiasis. Lactoplantibacillus species with proved with health
benefits are probiotics that improve the balance of microbiome in skin and gut.
In the present study, we investigated the potential antimicrobial activity of
Lactiplantibacillus plantarum APsulloc 331261 (APsulloc 331261) and
Lactiplantibacillus plantarum APsulloc 331266 (APsulloc 331266) derived
from green tea, in inhibiting five skin pathogenic strains
(Staphylococcus aureus (S. aureus), Cutibacterium acnes
(C. acnes), Candia albicans (C. albicans), Malassezia
globosa (M. globose), and Malassezia restricta (M. restricta))
associated with skin infection. Viability of
S. aureus, C. acnes, C. albicans, M. globosa, and M. restricta was inhibited by indirect co-culture with APsulloc 331261 or APsulloc
331266 at various ratios. Different concentrations of the cell-free conditioned
media (CM) derived from APsulloc 331261 or APsulloc 331266 inhibited the
vaibility of S. aureus, C. acnes, C. albicans, M. globosa
and M. restricta in a dose dependent manner. Moreover, susceptibility
of S. aureus, C. acnes, and C. albicans against APsulloc 331261
or APsulloc 331266 was confirmed following agar overlay methods. Results of the
agar overlay confirmed that various concentrations of APsulloc 331261 and
APsulloc 331266 exhibited low to high inhibitory activity on the growth of
S. aureus (ZDI 20.3