IMR Press / FBL / Volume 9 / Issue 3 / DOI: 10.2741/1386

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Article
Quantifying cell-adhesion strength with micropipette manipulation: principle and application
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1 Department of Biomedical Engineering, Washington University, Saint Louis, MO 63130, USA
Front. Biosci. (Landmark Ed) 2004, 9(3), 2183–2191; https://doi.org/10.2741/1386
Published: 1 September 2004
Abstract

Quantifying cell-adhesion strength is of great importance in biology and medicine. Cell-adhesion strength can be characterized by separating two adherent cells and determining the force required to do so, or by measuring the lifetime of a receptor-ligand bond that mediates cell adhesion. To this end, several micropipette-based experimental techniques that operate at both cellular and molecular levels have been developed over the past few decades. In this review, we provide an overview of three of these techniques, i.e., the step-pressure technique (SPT), the biomembrane-force probe (BFP), and the micropipette-aspiration technique (MAT). More detailed discussion will be given about the requirements and applications of the MAT.

Keywords
Cell biomechanics
Receptor-ligand kinetics
Tether formation
Single bond
Receptor anchorage
Adhesion energy
Review
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