IMR Press / FBL / Volume 9 / Issue 2 / DOI: 10.2741/1346

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Article
Applying high throughput techniques in the study of adenosine kinase in plant metabolism and development
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1 Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1
Front. Biosci. (Landmark Ed) 2004, 9(2), 1771–1781; https://doi.org/10.2741/1346
Published: 1 May 2004
Abstract

Adenosine kinase (ADK, EC 2.7.1.20) is a purine salvage enzyme, which phosphorylates adenosine (Ado) to AMP. It may also contribute to the interconversion of cytokinin ribosides and nucleotides. Recent microarray analyses have provided new insights into the impact of ADK activity towards plant metabolism and development. The majority of these findings reflect ADK's role in the metabolism of Ado produced from transmethylation reactions in addition to providing necessary nucleotides for the synthesis of nucleic acids and nucleotide cofactors. As such, ADK was found to increase during events associated with high transmethylation activity, such as cell wall synthesis and seed filling. Differences between plant organs were also detected, with ADK transcript levels found highest in siliques and roots and lowest in callus, leaves and buds. Transcript profiling of Arabidopsis expression using microarrays, reveals a predominance of ADK1 expression relative to that of ADK2. In the majority of the studies, the isoforms appeared to behave in a similar pattern of expression, with the exception being microgametogenesis where ADK1 was up-regulated when ADK2 was not. What specialized function the ADK1 could be providing to these cells during development and whether or not this is occurring in other biochemical processes has yet to be determined.

Keywords
ADK
Adenosine kinase
SAM
s-adenosylmethionine
SAM synthetase
SAHH
Methylation
Microarray
APT
Adenosine deaminase
Adenosine nucleosidase
Review
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