IMR Press / FBL / Volume 8 / Issue 6 / DOI: 10.2741/991

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Immunodiagnosis of ABPA
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1 Molecular Biochemistry and Diagnostics Division, Institute of Genomics and Integrative Biology, CSIR, Mall Road, Near Jubilee Hall, Delhi 110007, India
2 Allergy-Immunology Division, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, and V A Medical Center 151-I, 5000 West National Avenue, Milwaukee, WI 53295, USA

Academic Editor: Viswanath Kurup

Front. Biosci. (Landmark Ed) 2003, 8(6), 1187–1198;
Published: 1 September 2003
(This article belongs to the Special Issue Allergic aspergillosis)

For the diagnosis of allergic aspergillosis demonstration of specific immune responses to allergens has been accepted as a significant paradigm. Elevated levels of total IgE and Aspergillus fumigatus specific IgE and IgG antibodies are important criteria for diagnosis of ABPA. Although reference antigens or standardized methods are not available, there are a number of relevant recombinant antigens, which have been isolated in recent years. Several techniques have been employed in the demonstration of specific antibodies against antigens of Aspergillus fumigatus in the sera of patients. Of these methods, the widely followed ones are ELISA, radioimmunoassay, Western blot, and agar gel double diffusion. Recently, semi-automated methods have been developed using recombinant allergens to detect circulating antibody against Aspergillus. However, these methods have not been evaluated widely. Here we review the immunodiagnostic methods currently in use for allergic bronchopulmonary aspergillosis.

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